Journal: Genes

Article Title: Extracellular Polysaccharide Receptor and Receptor-Binding Proteins of the Rhodobacter capsulatus Bacteriophage-like Gene Transfer Agent RcGTA

doi: 10.3390/genes14051124

Figure Lengend Snippet: Gene transfer frequencies mediated by RcGTA. The relative number of strains 37b4 and 37b4(pCPS1) kanamycin-resistant colonies due to RcGTA-mediated transfer of locus rcc02539 that was disrupted by a kanamycin resistance cassette compared to strain SB1003. Blue bars represent the average of two technical replicates, and error bars show the range.

Article Snippet: Tetracycline-resistant colonies of 37b4 were resuspended to a turbidity of 140 Klett units and grown into the stationary phase, after which they were used in a gene transfer experiment with RcGTA obtained from the kanamycin-resistant strain DE2539, as described [ ].

Techniques:

Journal: Genes

Article Title: Extracellular Polysaccharide Receptor and Receptor-Binding Proteins of the Rhodobacter capsulatus Bacteriophage-like Gene Transfer Agent RcGTA

doi: 10.3390/genes14051124

Figure Lengend Snippet: Binding of RcGTA to cells. RcGTA was incubated with SB1003, 37b4, or 37b4(pCPS1) cells to allow binding, cell-bound RcGTA was removed by centrifugation of cells, and residual RcGTA recovered from the supernatant. The relative amount of unbound RcGTA is indicated by the number of kanamycin-resistant SB1003 colonies due to the RcGTA-mediated transfer of locus rcc02539 disrupted by a kanamycin resistance cassette. Blue bars represent the average of three biological replicates, and error bars show the standard deviation.

Article Snippet: Tetracycline-resistant colonies of 37b4 were resuspended to a turbidity of 140 Klett units and grown into the stationary phase, after which they were used in a gene transfer experiment with RcGTA obtained from the kanamycin-resistant strain DE2539, as described [ ].

Techniques: Binding Assay, Incubation, Centrifugation, Standard Deviation